LOCUS       pLV-[hsa-miR-520h]        9154 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7075..7935
                     /label=Amp(R)
     promoter        8136..8676
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5680..5914
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6489..6944)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5986..6116
                     /label=SV40 pA
     misc_feature    6117..6330
                     /label=SV40 ori
     misc_feature    5001..5597
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3619
                     /label=intron
     CDS             3666..4991
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3409
                     /label=hsa-mir-520h precursor
BASE COUNT     2181 a      2352 c      2481 g      2140 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gtccttttag 
     3121 gggtttatgt tctggattcc agaaaatatg caaacagggc caataaatgc atctttattt 
     3181 tgtgtgtatt ttaacctggt caaggaaaat tccaacaaga aacccagagt gctggagcaa 
     3241 gaagatccca tgctgtgacc ctctagagga agcactttct gtttgttgtc tgagaaaaaa 
     3301 caaagtgctt ccctttagag ttactgtttg ggaaaagcag tgttgaagtt gatgctgatg 
     3361 ttggtaatat atttgcatgc ttatcatcag acttggatga tggtggggtg ctagctcgag 
     3421 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca 
     3481 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga 
     3541 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag 
     3601 tttttttctt ccatttcagg tgtcgtgaaa actacccctc tagagtcgag ctaccggtcg 
     3661 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct 
     3721 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg 
     3781 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc 
     3841 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga 
     3901 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg 
     3961 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc 
     4021 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc 
     4081 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg 
     4141 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg 
     4201 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca 
     4261 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt 
     4321 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggacccac 
     4381 cggtcgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc 
     4441 ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg 
     4501 tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg 
     4561 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga 
     4621 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg 
     4681 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc 
     4741 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca 
     4801 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc 
     4861 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca 
     4921 ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg acccgcaagc 
     4981 ccggtgcctg agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt 
     5041 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc 
     5101 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg 
     5161 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac 
     5221 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc 
     5281 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc 
     5341 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa 
     5401 gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc 
     5461 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc 
     5521 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg 
     5581 ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca 
     5641 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc 
     5701 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga 
     5761 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 
     5821 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 
     5881 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt 
     5941 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca 
     6001 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 
     6061 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc 
     6121 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt 
     6181 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct 
     6241 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc 
     6301 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg 
     6361 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 
     6421 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg 
     6481 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag 
     6541 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt 
     6601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt 
     6661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg 
     6721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt 
     6781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt 
     6841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca 
     6901 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg 
     6961 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 
     7021 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 
     7081 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 
     7141 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 
     7201 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 
     7261 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 
     7321 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 
     7381 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 
     7441 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 
     7501 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 
     7561 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 
     7621 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 
     7681 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 
     7741 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 
     7801 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 
     7861 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 
     7921 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 
     7981 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     8041 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     8101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     8161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     8221 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 
     8281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     8341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     8401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     8461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     8521 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     8581 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     8641 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     8701 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 
     8761 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 
     8821 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 
     8881 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 
     8941 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact 
     9001 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg 
     9061 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt 
     9121 aaccctcact aaagggaaca aaagctggag ctgc 
//
